An Unbiased View of how HPLC works
An Unbiased View of how HPLC works
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, by way of example, demonstrates an amperometric stream mobile. Effluent from your column passes above the working electrode—held at a relentless possible relative to your downstream reference electrode—that absolutely oxidizes or decreases the analytes.
각각 다른 산업 분야에 대한 자세한 정보 및 다양한 카테고리는 다음 써모 피셔 사이언티픽 학습 센터에서 산업 및 응용 과학 페이지를 확인하세요.
The solvent reservoir holds the cellular phase, a liquid or solvent combination that continually flows from the HPLC system. The cell phase performs a vital job in separating sample components.
are made by reacting the silica particles with an organochlorosilane of the general variety Si(CH3)2RCl, exactly where R can be an alkyl or substituted alkyl team.
Distinctive solvents have various polarities, which impact their interaction Using the stationary section and eventually have an effect on the separation of analytes. Common solvents Utilized in HPLC include things like:
Bubbling an inert fuel with the cell period releases risky dissolved gases. This process known as sparging.
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前述した従来の順相タイプに対して、逆相クロマトグラフィーにおいては固定相に低極性のもの（例えばシリカゲルにアルキル基を共有結合させたもの）を、移動相に高極性のもの（例えば水や塩類の水溶液、アルコール、アセトニトリルなどの有機溶媒）を用いる。また珍しいケースではあるが、分離のための移動相pHをシリカゲルの使用範囲から外れたところに設定する必要がある場合、あるいはシリカゲル表面に残っている未反応シラノール基が分離に悪影響を及ぼし、かつそれが移動相の変更によっても解決できない場合には、固定相として樹脂を用いることがある。分析物はより極性の低いほどより強く固定相と相互作用して溶出が遅くなる。また極性の低い物質の割合が多い移動相ほど溶出が早くなる。
four. In the event the peaks for fluoxetine and protriptyline are resolved insufficiently, how may possibly you change the cellular stage to further improve their separation?
-hydroxybenzoic acid (PH) on a nonpolar C18 column matter to the optimum Evaluation time of six min. The shaded locations depict locations exactly where a separation is impossible, Using the unresolved solutes discovered.
*본 포스팅의 저작권은 써모 피셔 사이언티픽에 있으며, 콘텐츠의 무단 복제 및 수정, 재배포를 금지합니다.
Within the ionization chamber the remaining molecules—a mix from the cell stage components and solutes—go through ionization and fragmentation. The mass spectrometer’s mass analyzer separates the ions by their mass-to-charge ratio (m/z). A detector counts the ions and displays the mass spectrum.
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, which can be the greater prevalent kind of HPLC, the stationary phase is nonpolar plus the mobile section is polar. The most common nonpolar stationary phases use an organochlorosilane in which the R team is undoubtedly an n